RNA-cap related proteins and development of high throughput inhibitor screening approaches
Proteins responsible for nucleotide binding and enzymatic transformations are often therapeutic targets. We are interested in biologically and therapeutically relevant cap-recognizing proteins related to mRNA function and mRNA turnover, including eukaryotic translation initiation factor 4E, decapping enzymes, RNA methyltransferases and many others. To facilitate studies on those proteins and discovery of their specific agonists or inhibitors, we develop different binding and activity assays based on specifically tailored molecular probes. In inhibitor discovery we rely both on structure-guided design and random screening approaches.