19F nuclei have several favourable properties for NMR applications, including nuclear spin equal 1/2, high magneto-gyric coefficient, large chemical shift dispersion and 100%, abundance, which altogether result in high sensitivity and utility of 19F NMR measurements. Consequently, fluorine-labeled compounds and their transformations can be selectively observed by 19F NMR even in complex biological mixtures. Our group has been developing 19F-labeled nucleotides and nucleic acids for wide range of applications in biophysical and biochemical 19F NMR experiments, including enzymatic assays for phosphohydrolases and sulfotransferases and structural and functional studies on nucleic acids.

Selected publications:

https://pubs.acs.org/doi/abs/10.1021/acschembio.1c00978

https://academic.oup.com/nar/article/48/15/8209/5854145?login=true

https://pubs.acs.org/doi/abs/10.1021/acs.joc.9b03198

https://pubs.acs.org/doi/abs/10.1021/acs.joc.5b00337