OUR PUBLICATIONS

SELECTED PUBLICATIONS

Ethylenediamine derivatives efficiently react with oxidized RNA 3′ ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation

By A. Mamot, P.J. Sikorski, A. Siekierska, P. de Witte, J. Kowalska, J. Jemielity;  Nucleic Acids Research 50, e3-e3

5′-fluoro(di)phosphate-labeled oligonucleotides are versatile molecular probes for studying nucleic acid secondary structure and interactions by 19F NMR

By M. R. Baranowski, M. Warminski, J. Jemielity, J. Kowalska;  Nucleic Acids Research 48, 8209-8224.

5′-Phosphorothiolate Dinucleotide Cap Analogues: Reagents for Messenger RNA Modification and Potent Small-Molecular Inhibitors of Decapping Enzymes

By B. A. Wojtczak, P. J. Sikorski, K. Fac-Dąbrowska, A. Nowicka, M. Warmiński, D. Kubacka, E. Nowak, M. Nowotny, J. Kowalska, J. Jemielity; Journal of the American Chemical Society 140, 5987-5999

Cellular delivery of dinucleotides by conjugation with small molecules: Targeting translation initiation for anticancer applications

By N Kleczewska, PJ Sikorski, Z Warminska, L Markiewicz, R Kasprzyk, N. Baran, K. Kwapiszewska, A. Karpinska, J. Michalski, R. Holyst, J. Kowalska, J. Jemielity; Chemical Science 12, 10242-10251.

The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5′ cap modulates protein expression in living cells

By P. J. Sikorski, M. Warminski, D. Kubacka, T. Ratajczak, D. Nowis, J. Kowalska, J.Jemielity; Nucleic Acids Research 48, 1607-1626.

Azido-Functionalized 5ʹ-Cap Analogs for Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells

By Mamot A., Sikorski P. J., Warminski M., Kowalska J., Jemielity J; Angewandte Chemie International Edition 56, 15628–15632

2022

2021

R Kasprzyk, J Jemielity; Enzymatic assays to explore viral mRNA capping machinery. ChemBioChem 22, 3236-3253 (Very Important Paper)
R. Wojcik, M. R. Baranowski, L. Markiewicz, D. Kubacka, M. Bednarczyk, N. Baran, A. Wojtczak, P. J Sikorski, J. Zuberek, J. Kowalska, J. Jemielity; Novel N7-Arylmethyl Substituted Dinucleotide mRNA 5′ cap Analogs: Synthesis and Evaluation as Modulators of Translation. Pharmaceutics 13, 1941
R. Kasprzyk, T.J. Spiewla, M. Smietanski, S. Golojuch, L. Vangeel, S. De Jonghe, D. Jochmans, J. Neyts, J. Kowalska, J. Jemielity; Identification and evaluation of potential SARS-CoV-2 antiviral agents targeting mRNA cap guanine N7-Methyltransferase. Antiviral Research 193, 105142.
A. Depaix, A. Mlynarska‐Cieslak, M. Warminski, P.J. Sikorski, J. Jemielity, J. Kowalska; RNA Ligation for Mono and Dually Labeled RNAs. Chemistry–A European Journal 27, 12190-12197
O. Perzanowska, M. Majewski, M. Strenkowska, P. Głowala, M. Czarnocki-Cieciura, M. Mazur, J. Kowalska, J. Jemielity; Nucleotide-decorated AuNPs as probes for nucleotide-binding proteins. Scientific Reports 11, Art. no. 15741.
A. Galloway, A. Kaskar, D. Ditsova, A. Atrih, H. Yoshikawa, C. Gomez-Moreira, O. Suska, M. Warminski, R. Grzela, A.I. Lamond, E. Darzynkiewicz, J. Jemielity, V. H. Cowling; Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation. Nucleic Acids Research 49, 6722-6738.
R.W. Tibble, A. Depaix, J. Kowalska, J. Jemielity, J.D. Gross; Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation. Nature Chemical Biology 17, 615-623.
A. Wojtczak, R. Kasprzyk, M. Warmiński, K. Ubych, D. Kubacka, P.J. Sikorski, J. Jemielity, J. Kowalska; Evaluation of carboxyfluorescein-labeled 7-methylguanine nucleotides as probes for studying cap-binding proteins by fluorescence anisotropy. Scientific Reports 11, Art, no. 1184.
P. Rzymski, L. Borkowski, M. Drąg, R. Flisiak, J. Jemielity, J. Krajewski, A. Mastalerz-Migas, A. Matyja, K. Pyrć, K. Simon, M. Sutkowski, J. Wysocki, J. Zajkowska, A. Fal; The strategies to support the COVID-19 vaccination with evidence-based communication and tackling misinformation. Vaccines 9, Art. no. 109
M. Warminski, J. Kowalska, E. Nowak, D. Kubacka, R. Tibble, R. Kasprzyk, P. J. Sikorski, J. D. Gross, M. Nowotny, J. Jemielity Structural Insights into the Interaction of Clinically Relevant Phosphorothioate mRNA Cap Analogs with Translation Initiation Factor 4E Reveal Stabilization via Electrostatic Thio-Effect. ACS Chemical Biology 16, 334-343.
N. Kleczewska, P.J. Sikorski, Z. Warminska, L. Markiewicz, R. Kasprzyk, N. Baran, K. Kwapiszewska, A. Karpinska, J. Michalski, R. Holyst, J. Kowalska, J. Jemielity; Cellular delivery of dinucleotides by conjugation with small molecules: Targeting translation initiation for anticancer applications. Chemical Science 12, 10242-10251.

 

2020

D. Strzelecka, M. Smietanski, P. J. Sikorski, M. Warminski, J. Kowalska, J. Jemielity; Phosphodiester modifications in mRNA poly (A) tail prevent deadenylation without compromising protein expression. RNA 26 (12), 1815-1837.

M. R. Baranowski, M. Warminski, J. Jemielity, J. Kowalska; 5′-fluoro(di)phosphate-labeled oligonucleotides are versatile molecular probes for studying nucleic acid secondary structure and interactions by 19F NMR. Nucleic Acids Research 48, 8209-8224.

M. Chrominski, J. Kowalska, J. Jemielity; Efficient Synthesis of Trifluoromethylated Purine Ribonucleosides and Ribonucleotides. Current Protocols in Nucleic Acid Chemistry 83, e118.

M. Warminski, J. Kowalska, J. Jemielity; Solid‐Phase Synthesis of RNA 5′‐Azides and Their Application for Labeling, Ligation, and Cyclization Via Click Chemistry. Current Protocols in Nucleic Acid Chemistry 82, e112.

R. Kasprzyk, M. Fido, A. Mamot, P. Wanat, M. Smietanski, M. Kopcial, V. H. Cowling, J. Kowalska, J. Jemielity; Direct High‐Throughput Screening Assay for mRNA Cap Guanine‐N7 Methyltransferase Activity. Chemistry–A European Journal 26, 11266-11275.

S. Golojuch, M. Kopcial, D. Strzelecka, R. Kasprzyk, N. Baran, P. J. Sikorski, , J. Kowalska, J. Jemielity; Exploring tryptamine conjugates as pronucleotides of phosphate-modified 7-methylguanine nucleotides targeting cap-dependent translation. Bioorganic & Medicinal Chemistry 28, 115523.

P. J. Sikorski, M. Warminski, D. Kubacka, T. Ratajczak, D. Nowis, J. Kowalska, J.Jemielity; The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5′ cap modulates protein expression in living cells. Nucleic Acids Research 48, 1607-1626.

M. Pietrowska-Borek, A. Wojdyła-Mamoń, J. Dobrogojski, A. Młynarska-Cieślak, M. R. Baranowski, J. M. Dąbrowski, J. Kowalska, J. Jemielity, S. Borek, M. Angeles Pedreño, A. Guranowski; Purine and pyrimidine dinucleoside polyphosphates differentially affect the phenylpropanoid pathway in Vitis vinifera L. cv. Monastrell suspension cultured cells. Plant Physiology and Biochemistry 147, 125-132.

M. Chrominski, M.R. Baranowski, S. Chmielinski, J. Kowalska, J. Jemielity; Synthesis of Trifluoromethylated Purine Ribonucleotides and Their Evaluation as 19F NMR Probes. The Journal of Organic Chemistry 85, 3440-3453.

B. Miedziak, A. Dobieżyńska, Z. M. Darżynkiewicz, J. Bartkowska, J. Miszkiewicz, J. Kowalska, M. Warminski, M. Tyras, J. Trylska, J. Jemielity, E. Darzynkiewicz, R. Grzela; Kinetic analysis of IFIT1 and IFIT5 interactions with different native and engineered RNAs and its consequences for designing mRNA-based therapeutics. RNA 26, 58-68.

 

2019

R. Kasprzyk, B. J. Starek, S. Ciechanowicz, D. Kubacka, J. Kowalska, J. Jemielity; Fluorescent Turn‐On Probes for the Development of Binding and Hydrolytic Activity Assays for mRNA Cap‐Recognizing Proteins. Chemistry–A European Journal 25, 6728-6740.

M. Kopcial, B. A. Wojtczak, R. Kasprzyk, J. Kowalska, J. Jemielity; N1-Propargylguanosine Modified mRNA Cap Analogs: Synthesis, reactivity, and applications to the study of cap-binding proteins. Molecules 24, 1899.

 

2018

B. A. Wojtczak, P. J. Sikorski, K. Fac-Dąbrowska, A. Nowicka, M. Warmiński, D. Kubacka, E. Nowak, M. Nowotny, J. Kowalska, J. Jemielity. 5′-Phosphorothiolate Dinucleotide Cap Analogues: Reagents for Messenger RNA Modification and Potent Small-Molecular Inhibitors of Decapping Enzymes. Journal of the American Chemical Society 140, 5987-5999 

Mlynarska-Cieślak A., Depaix A., Grudzien-Nogalska E., Sikorski P.J., Warminski M., Kiledjian M., Jemielity J., Kowalska J. Nicotinamide Containing Di- and Trinucleotides as Chemical Tools for Studies of NAD-Capped RNAs. Organic Letters 20, 7650–7655

Bednarek S., Madan V., Sikorski P.J., Bertenschlager R., Kowalska J., Jemielity J. mRNAs biotinylated within the 5’ cap and protected against decapping: new tools to capture RNA – protein complexes. Philosophical Transactions of the Royal Society B – Biological Sciences 373, Article ID:20180167

Mugridge J.S., Tibble R.W., Ziemniak M., Jemielity J., Gross J.D. Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis. Nature Communications 9, Art. No.1152

Walczak S., Sikorski P. J., Kasprzyk R., Kowalska J., Jemielity J. Exploring the potential of phosphotriazole 5′ mRNA cap analogues as efficient translation initiators. Organic and Biomolecular Chemistry 16, 6741-6748

Wanat P., Kasprzyk R., Kopciał M., Sikorski P. J., Strzelecka D., Jemielity J., Kowalska J., ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions. Chemical Communications 54, 9773-9776

Essig K., Kronbeck N., Guimaraes J. C., Lohs C., Schlundt A., Hoffmann A., Behrens G., G. Brenner G., Kowalska J., Lopez-Rodriguez C., Jemielity J., Holtmann H., Reiche K., Hackermüller J., Sattler M., Zavolan M., Heissmeyer V. Roquin targets mRNAs in a 3′-UTR-specific manner by different modes of regulation. Nature Communications 9 Art. No. 3810

 

2017

Mamot A., Sikorski P. J., Warminski M., Kowalska J., Jemielity J.. Azido-Functionalized 5ʹ-Cap Analogs for Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells. Angewandte Chemie International Edition 56, 15628–15632

Rydzik A. M., Warminski M., Sikorski P. J., Baranowski M. R., Walczak S., Kowalska J., Zuberek J., Lukaszewicz M., Nowak E., Claridge T. D. W., Darzynkiewicz E., Nowotny M., Jemielity J..  mRNA cap analogues substituted in the tetraphosphate chain with CX2: identification of O-to-CCl2 as the first bridging modification that confers resistance to decapping without impairing translation. Nucleic Acids Research 45, 8661-8675 

Walczak S., Nowicka A., Kubacka D., Fac K., Wanat P., Mroczek S., Kowalska J., Jemielity J.. A novel route for preparing 5′ cap mimics and capped RNAs: phosphate-modified cap analogues obtained via click chemistry. Chemical Science 8, 260 – 267 

Warminski M., Kowalska J., Jemielity J., Synthesis of RNA 5′-Azides from 2′-O-Pivaloyloxymethyl-Protected RNAs and Their Reactivity in Azide–Alkyne Cycloaddition Reactions. Organic Letters 19,  3624–3627

Warminski M., Sikorski P. J., Warminska Z., Lukaszewicz M., Kropiwnicka A., Zuberek J., Darzynkiewicz E., Kowalska J., Jemielity J., Amino-functionalized 5’ cap analogs as tools for site-specific sequence-independent labeling of messenger RNA. Bioconjugate Chemistry 28, 1978-1992

Strzelecka D., Chmielinski S., Bednarek S., Jemielity J., Kowalska J.. Analysis of mononucleotides by tandem mass spectrometry: investigation of fragmentation pathways for phosphate- and ribose-modified nucleotide analogues. Scientific Reports 7  Art. No. 8931

Warminski M., Sikorski P. J., Kowalska J., Jemielity J. . Applications of Phosphate Modification and Labeling to Study (m)RNA Caps. Topics in Current Chemistry 375 Art no. 16.

Min K.-W., Davila S., Zealy R. W., Lloyd L. T., Lee I. Y., Lee R., Roh K. H., Jung A., Jemielity J., Choi E.-J., Yoon J.-H., eIF4E phosphorylation by MST1 reduces translation of a subset of mRNAs, but increases lncRNA translation. Biochimica et Biophysica Acta – Gene Regulatory Mechanisms 1860, 761–772

B. Kroczynska, G. T. Blyth, R. L. Rafidi, B. Majchrzak-Kita, L. Xu, D. Saleiro, E. M. Kosciuczuk, J. Jemielity, B. Su, J. K. Altman, E. A. Eklund, E. N. Fish, L. C. Platanias. Central Regulatory Role for SIN1 in Interferon γ (IFNγ) Signaling and Generation of Biological Responses. J. Biol. Chem. 292, 4743-4752

 

2016

Strenkowska M., Grzela R., Majewski M., Wnek K., Kowalska J., Lukaszewicz M., Zuberek J., Darzynkiewicz E., Kuhn A. N., Sahin U., Jemielity J., Cap analogs modified with 1,2-dithiodiphosphate moiety protect mRNA from decapping and enhance its translational potential, Nucleic Acids Research 44, 9578-9590(IF 10.162)

Mugridge J. S., Ziemniak M., Jemielity J., Gross J. D., Structural basis of mRNA-cap recognition by Dcp1–Dcp2, Nature Structural & Molecular Biology 23, 987–994

Honcharenko M., Bestas B., Jezowska M., Wojtczak B. A., Moreno P., Romanowska J., Bächle S.M., Darzynkiewicz E., Jemielity J., Smith C.I.E., Stromberg R., Synthetic m3G-CAP attachment necessitates a minimum trinucleotide constituent to be recognized as a Nuclear Import Signal, RSC Advances 6, 51367-51373

Baranowski M., Nowicka A., Jemielity J., Kowalska J., Fluorescent HTS assay for phosphohydrolases based on nucleoside 5ʹ-fluorophosphates: application in screening for inhibitors of mRNA Decapping Scavenger and PDE-I, Organic & Biomolecular Chemistry 14, 4595-4604

Kasprzyk R., Kowalska J., Wieczorek Z., Szabelski M., Stolarski R., Jemielity J., Acetylpyrene-labelled 7-methylguanine nucleotides: unusual fluorescence properties and application to Decapping Scavenger activity monitoring, Organic & Biomolecular Chemistry 14, 3863-3868 

Ziemniak M., Mugridge J.S., Kowalska J., Rhoads R.E., Gross J.D., Jemielity J., Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex, RNA 22, 518-529

Wojtczak B.A., Warminski M., Kowalska J., Lukaszewicz M., Honcharenko M., Smith C. I.E., Stromberg R., Darzynkiewicz E., Jemielity J., Clickable trimethylguanosine cap analogs modified within the triphosphate bridge: synthesis, conjugation to RNA and susceptibility to degradation, RSC Advances 6, 8317-8328 

Kroczynska B., Rafidi R.L., Majchrzak-Kita B., Kosciuczuk E.M., Blyth G.T., Jacek Jemielity J., Warminska Z., Saleiro D., Mehrotra S., Arslan A.D., Fish E.N., Platanias L.C., Interferon γ (IFNγ)-Signaling via mTORC2 and Regulatory Effects in the Generation of Type II IFN Biological Responses, J. Biol. Chem. 291, 2389–2396

 

2015

Ziemniak M., Kowalska J., Lukaszewicz M., Zuberek J., Wnek K., Darzynkiewicz E., Jemielity J., Phosphate-modified analogues of m7GTP and m7Gppppm7G – synthesis and biochemical properties, Bioorganic & Medicinal Chemistry 23, 5369–5381

Warminski M., Warminska Z., Kowalska J., Jemielity J., mRNA Cap Modification through Carb­amate Chemistry: Synthesis of Amino- and Carboxy-Functionalised Cap Analogues Suitable for Labelling and Bioconjugation, European Journal of Organic Chemistry 2015, 6153–6169 

Wanat P., Walczak S., Wojtczak B. A., Nowakowska M., Jemielity J., Kowalska J., Ethynyl, 2-Propynyl, and 3-Butynyl C-Phosphonate Analogues of Nucleoside Di- and Triphosphates: Synthesis and Reactivity in CuAAC, Organic Letters 17, 3062-3065

Baranowski M. R., Nowicka A., Rydzik A. M., Warminski M., Kasprzyk R., Wojtczak B. A., Wojcik J., Claridge T. D. W., Kowalska J., Jemielity J., Synthesis of Fluorophosphate Nucleotide Analogs and Their Characterization as Tools for 19F NMR Studies, The Journal of Organic Chemistry 80, 3982–3997

Kijewska K., Głowala P., Kowalska J., Jemielity J., Kaczyńska K., Janiszewska K., Stolarski J., Blanchard G.J., Kępińska D., Lubelska K., Wiktorska K., Pisarek M., Mazur M., Gold-decorated polymer vessel structures as carriers of mRNA cap analogs, Polymer 57, 77-87

Kropiwnicka A., Kuchta K., Lukaszewicz M., Kowalska J., Jemielity J., Ginalski K., Darzynkiewicz E., Zuberek J., Five eIF4E isoforms from Arabidopsis thaliana are characterized by distinct features of cap analogs binding, Biochemical and Biophysical Research Communications 456, 47-52

2014

Zochowska M., Piguet A. C., Jemielity J., Kowalska J., Szolajska E., Dufour J. F., Chroboczek J., Virus-like particle-mediated intracellular delivery of mRNA cap analog with in vivo activity against hepatocellular carcinoma, Nanomedicine: Nanotechnology, Biology and Medicine 11, 67-76 

Zytek M., Kowalska J., Lukaszewicz M., Wojtczak B. A., Zuberek J., Ferenc-Mrozek A., Darzynkiewicz E., Niedzwiecka A., Jemielity J., Towards novel efficient and stable nuclear import signals: synthesis and properties of trimethylguanosine cap analogs modified within the 5’,5’-triphosphate bridge, Org. Biomol. Chem. 12, 9184 – 9199

Kowalska J., Wypijewska del Nogal A., Darzynkiewicz Z. M., Buck J., Nicola C.,  Kuhn A., Lukaszewicz M., Zuberek J., Strenkowska M., Ziemniak M., Maciejczyk M., Bojarska E., Rhoads R. E., Darzynkiewicz E., Sahin U., Jemielity J., Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes, Nucleic Acids Research 42, 10245-10264 (IF 10.162)

Nowakowska M., Kowalska J., Martin F., d’Orchymont A., Żuberek J., Łukaszewicz M., Darżynkiewicz E., Jemielity J., Cap analogs containing 6-thioguanosine – reagents for the synthesis of mRNAs selectively photo-crosslinkable with cap-binding biomolecules, Organic & Biomolecular Chemistry 12, 4841-4847

 

2013

Honcharenko M., Zytek M., Bestas B., Moreno P., Jemielity J., Darzynkiewicz E., Smith C.I. E., Strömberg R., Synthesis and evaluation of stability of m3G-CAP analogues in serum-supplemented medium and cytosolic extract, Bioorganic & Medicinal Chemistry 21, 7921–7928 

Wypijewska del Nogal A., Surleac M. D., Kowalska J., Lukaszewicz M., Jemielity J., Bisaillon M., Darzynkiewicz E., Milac A.L., Bojarska E., Analysis of Decapping Scavenger (DcpS)-cap complex using modified cap analogs reveals molecular determinants for efficient cap binding, FEBS Journal 280, 6508–6527

Ziemniak M., Szabelski M., Lukaszewicz M., Nowicka A., Darzynkiewicz E., Wieczorek Z., Rhoads R. E., Jemielity J., Synthesis and evaluation of fluorescent cap analogues for mRNA labeling, RSC Advances 3, 20943-20958 

Bretes E., Wojdyła-Mamoń A. M., Kowalska J., Jemielity J., Kaczmarek R., Baraniak J., Guranowski A., Hint2, the mitochondrial nucleoside 5′-phosphoramidate hydrolase; properties of the homogeneous protein from sheep (Ovis aries) liver, Acta Biochimica Polonica 60, 249-254 

Warminski M., Kowalska J., Buck J., Zuberek J., Lukaszewicz M., Nicola C., Kuhn A. N., Sahin U., Darzynkiewicz E., Jemielity J., The synthesis of isopropylidene mRNA cap analogs modified with phosphorothioate moiety and their evaluation as promoters of mRNA translation, Bioorganic & Medicinal Chemistry Letters 23, 3753–3758

Kijewska K., Jarzębińska A., Kowalska J., Jemielity J., Kępińska D., Szczytko J., Pisarek M., Wiktorska K., Stolarski J., Krysinski P. G., Twardowski A., Mazur M., Magnetic-nanoparticle-decorated polypyrrole microvessels: Toward encapsulation of mRNA cap analogs, Biomacromolecules 14, 1867−1876

Ziemniak M., Strenkowska M., Kowalska J., Jemielity J., Potential therapeutic applications of RNA cap analogs, Future Medicinal Chemistry 5, 1141-1172

Dabrowski-Tumanski P., Kowalska J., Jemielity J. ,Efficient and rapid synthesis of nucleoside diphosphate sugars from nucleoside phosphorimidazolides, Eur. J. Org. Chem. 2013, 2147 – 2154

Su W., Slepenkov S. V., Slevin M. K., Lyons S. M., Ziemniak M., Kowalska J., Darzynkiewicz E., Jemielity J., Marzluff W. F., Rhoads R. E., mRNAs containing the histone 3′ stem–loop are degraded primarily by decapping mediated by oligouridylation of the 3′ end, RNA 19, 1-16 

 

2012

Jemielity J., Lukaszewicz M., Kowalska J., Czarnecki J., Zuberek J., Darzynkiewicz E., Synthesis of biotin labelled cap analogue – incorporable into mRNA transcripts and promoting cap-dependent translation, Org. Biomol. Chem.  10, 8570-8574 

Strenkowska M., Wanat P., Ziemniak M., Jemielity J., Kowalska J., Preparation of Synthetically Challenging Nucleotides Using Cyanoethyl P-Imidazolides and Microwaves, Organic Letters 14, 4782-4785

Kowalska J., Osowniak A., Zuberek J., Jemielity J., Synthesis of nucleoside phosphosulfates, Bioorganic & Medicinal Chemistry Letters 22, 3661-3664

Szczepaniak S. A., Zuberek J., Darzynkiewicz E., Kufel J., Jemielity J., Affinity resins containing enzymatically resistant mRNA cap analogs-a new tool for the analysis of cap-binding proteins, RNA 18, 1421-1432 

Wypijewska A., Bojarska E., Lukaszewicz M., Stepinski J., Jemielity J.,. Davis R.E, Darzynkiewicz E., 7-methylguanosine diphosphate (m7GDP) is not hydrolyzed but strongly bound by decapping scavenger (DcpS) enzymes and potently inhibits their activity, Biochemistry 51, 8003-8013 

Rydzik, A. M., Kulis, M., Lukaszewicz, M., Kowalska, J. , Zuberek, J., Darzynkiewicz, Z. M., Darzynkiewicz, E., Jemielity, J., Synthesis and properties of mRNA cap analogs containing imidodiphosphate moiety – fairly mimicking natural cap structure, yet resistant to enzymatic hydrolysis, Bioorganic and Medicinal Chemistry 20, 1699-1710 

 

2011

Su, W., Slepenkov, S., Grudzien-Nogalska, E., Kowalska, J., Kulis, M., Zuberek, J., Lukaszewicz, M., Darzynkiewicz, E., Jemielity, J., Rhoads, R.E. Translation, stability, and resistance to decapping of mRNAs containing caps substituted in the triphosphate chain with BH(3), Se, and NH. RNA 17, 978-988

Frank F., Fabian M.R., Stepinski J., Jemielity, J., Darzynkiewicz, E., Sonenberg, N., Nagar B., Structural analysis of 5 ‘-mRNA-cap interactions with the human AGO2 MID domain. EMBO Reports 12, 415-420

Guranowski A.,Wojdyla A.M., Rydzik A.M. , Stepinski J., Jemielity J., Plant nucleoside 5 ‘-phosphoramidate hydrolase; simple purification from yellow lupin (Lupinus luteus) seeds and properties of homogeneous enzyme. Acta Biochimica Polonica 58, 131-136

 

2010

Kuhn A.N., Diken M., Kreiter S., Selmi A., Kowalska, J. Jemielity, J., Darzynkiewicz, E., Huber, C., Tureci O., Sahin, U. Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo. Gene Therapy 17, 961-971

Jemielity, J. Kowalska, J., Rydzik, A. M., Darzynkiewicz, E., Synthetic mRNA cap analogs with modified triphosphate bridge – synthesis, applications and prospects. New J. Chem. 34, 829-844 

Strenkowska M., Kowalska J., Lukaszewicz M., Zuberek J., Su W.,. Rhoads R. E, Darzynkiewicz E., Jemielity J., Towards mRNA with superior translational activity: synthesis and properties of ARCA tetraphosphates with single phosphorothioate modifications, New J. Chem. 34, 993-1007 

Wypijewska A., Bojarska E., Stepinski, J., Jankowska-Anyszka, M., Jemielity J., Davis, R.E., Darzynkiewicz E. Structural requirements for Caenorhabditis elegans DcpS substrates based on fluorescence and HPLC enzyme kinetic studies. FEBS J. 277, 3003-3013 

Guranowski A, Wojdyla A.M., Zimny J., Wypijewska A., Kowalska J., Jemielity J., Davis, R.E., Bieganowski P. Dual activity of certain HIT-proteins: A. thaliana Hint4 and C-elegans DcpS act on adenosine 5 ‘-phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP). FEBS Lett. 584, 93-98

Guranowski A., Wojdyla A.M., Zimny J., Wypijewska A., Kowalska J., Lukaszewicz M., Jemielity J., Darzynkiewicz E., Jagiello A., Bieganowski P. Recognition of different nucleotidyl-derivatives as substrates of reactions catalyzed by various HIT-proteins. New J. Chem. 34, 888-893

 

2009

 Kowalska J., Lukaszewicz M., Zuberek J., Darzynkiewicz E., Jemielity J.; Phosphoroselenoate Dinucleotides for Modification of mRNA 5′ End. ChemBioChem 10, 2469-2473
Rydzik A.M., Lukaszewicz M., Zuberek J., Kowalska J., Darzynkiewicz Z.M., Darzynkiewicz E., Jemielity J.; Synthetic dinucleotide mRNA cap analogs with tetraphosphate 5,5 bridge containing methylenebis(phosphonate) modification Org. Biomol. Chem. 7, 4763-4776
Zdanowicz A., Thermann R., Kowalska J., Jemielity J., Duncan K., Preiss T., Darzynkiewicz E., Hentze M.W.; Drosophila miR2 Primarily Targets the m7GpppN Cap Structure for Translational Repression. Molec. Cell 35, 881-888
Kowalska J, Lukaszewicz M, Zuberek J, Ziemniak M, Darzynkiewicz E, Jemielity J.; Phosphorothioate analogs of m7GTP are enzymatically stable inhibitors of cap-dependent translation. Bioorg. Med. Chem. Lett. 19, 1921-1925
Banerjee H., Palenchar J.B., Lukaszewicz M., Bojarska E., Stepinski J., Jemielity J., Guranowski A., Ng S., Wah D.A., Darzynkiewicz E., Bellofatto V.; Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: Substrate specificity studies on the recombinant and endogenous proteins. RNA 15, 1554-1564

 

2008

Tölle M, Jankowski V, Schuchardt M, Wiedon A, Huang T, Hub F, Kowalska J, Jemielity J, Guranowski A, Loddenkemper C, Zidek W, Jankowski J, van der Giet M.Adenosine 5′-Tetraphosphate Is a Highly Potent Purinergic Endothelium-Derived Vasoconstrictor. Circulation Research 108, 1100 – 1108

Kowalska J., Lewdorowicz M., Zuberek J., Grudzien-Nogalska E., Bojarska E., Stepinski J., Rhoads R.E., Darzynkiewicz E., Davis R.E., Jemielity J.; Synthesis and Characterization of mRNA Cap Analogs Containing Phosphorothioate Substitutions that Bind Tightly to eIF4E and are Resistant to the Decapping Pyrophosphatase DcpS. RNA 14, 1119-1131

Deshmukh MV, Jones BN, Quang-Dang D-U, Flinders J, Floor SN, Kim C, Jemielity J, Kalek M, Darzynkiewicz E, Gross JD.; mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2. Molec. Cell 29, 324-336 

 

2007

Grudzien-Nogalska E, Jemielity J, Kowalska J, Darzynkiewicz E, Rhoads RE.; Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells. RNA 13, 1745-1755

Kowalska J, Lewdorowicz M, Darzynkiewicz E, Jemielity J.; A simple and rapid synthesis of nucleotide analogues containing a phosphorothioate moiety at the terminal position of the phosphate chain. Tetrahedron Lett. 48, 5475-5479

Grudzien-Nogalska E, Stepinski J, Jemielity J, Zuberek J, Stolarski R, Rhoads RE, Darzynkiewicz E.; Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability. Method Enzymol. 431, 203-227

Darzynkiewicz ZM, Bojarska E, Kowalska J, Lewdorowicz M, Jemielity J, Kalek M, Stepinski J, Davis RE, Darzynkiewicz E.; Interaction of human decapping scavenger with 5 ‘ mRNA cap analogues: structural requirements for catalytic activity. J Phys-Condens Mat. 19 art. no. 285217

Zuberek J, Kubacka D, Jablonowska A, Jemielity J, Stepinski J, Sonenberg N, Darzynkiewicz E.; Weak binding affinity of human 4EHP for mRNA cap analogs. RNA 13, 691-697

Kowalska J, Lewdorowicz M, Zuberek J, Bojarska E, Stepinski J, Stolarski R, Darzynkiewicz E, Jemielity J.; Assignment of the absolute configuration of P-chiral 5’mRNA cap analogues containing phosphorothioate moiety. Nucleosides Nucleotides & Nucleic Acids 26, 1301-1305 

Jemielity J, Stolarski R, Darzynkiewicz E.; Synthesis of H-3 and C-13 labeled mRNA cap dinucleotides-useful tools for NMR, biochemical, and biological studies. Nucleosides Nucleotides & Nucleic Acids 26, 1315-1319

Lewdorowicz M, Jemielity J, Kierzek R, Shapira M, Stepinski J, Darzynkiewicz E.; Solid-supported synthesis of 5 ‘-mRNA cap-4 from trypanosomatids. Nucleosides Nucleotides & Nucleic Acids 26, 1329-1333

Wierzchowski J, Pietrzak M, Stepinski J, Jemielity J, Kalek M, Bojarska E, Jankowska-Anyszka M, Davis RE, Darzynkiewicz E.; Kinetics of C elegans DcpS cap hydrolysis studied by fluorescence spectroscopy. Nucleosides Nucleotides & Nucleic Acids 26, 1211-1215

Lewdorowicz M, Stepinski J, Kierzek R, Jemielity J, Zuberek J, Yoffe Y, Shapira M, Stolarski R, Darzynkiewicz E.; Synthesis of leishmania cap-4 intermediates, cap-2 and cap-3. Nucleosides Nucleotides & Nucleic Acids 26, 1339-1348

Darzynkiewicz ZM, Bojarska E, Stepinski J, Jemielity J, Jankowska-Anyszka M, Davis RE, Darzynkiewicz E.; Affinity of dinucleotide cap analogues for human decapping scavenger (hdcps). Nucleosides Nucleotides & Nucleic Acids 26, 1349-1352 (2007).

 

2006

Kalek M, Jemielity J, Darzynkiewicz ZM, Bojarska E, Stepinski J, Stolarski R, Davis RE, Darzynkiewicz E; Enzymatically Stable 5′ mRNA Cap Analogs: Synthesis and Binding Studies with Human DcpS Decapping Enzyme. Bioorganic and Medicinal Chemistry 14, 3223-3230 (2006).

Guranowski A, Starzyńska E, Pietrowska-Borek M, Jemielity J, Kowalska J, Darzynkiewicz E, Thompson MJ, Blackburn MG; Methylene analogues of adenosine-5′-tetraphosphate: their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases. FEBS Journal 273, 829-838

Grudzien E, Kalek M., Jemielity J, Darzynkiewicz E, Rhoads RE; Differential inhibition of mRNA degradation pathways by novel cap analogs. Journal of Biological Chemistry 281, 1857-186

 

2005

Westman B, Beeren L, Grudzien E, Stepinski J, Worch R, Zuberek J, Jemielity J, Stolarski R, Darzynkiewicz E, Rhoads RE, Preiss T; The antiviral drug ribavirin does not mimic the 7-methylguanosine moiety of the mRNA cap structure in vitro. RNA 11, 1505-1513

Kowalska J, Lewdorowicz M, Zuberek J, Bojarska E, Wojcik J, Cohen LS, Davis RE, Stepinski J, Stolarski R, Darzynkiewicz E, Jemielity J; Synthesis of mRNA cap analogues containing phosphorothioate moiety in 5′,5′-triphosphate chain. Nucleosides Nucleotides & Nucleic Acids 24, 595-600

Jemielity J, Heinonen P, Lönnberg H, Darzynkiewicz E; Novel approach to solid phase chemical synthesis of oligonucleotide mRNA cap analogues, Nucleosides Nucleotides & Nucleic Acids 24, 601-605

Kalek M, Jemielity J, Zuberek J, Grudzień E, Bojarska E, Cohen LS, Stepinski J, Stolarski R, Davis RE, Rhoads RE, Darzynkiewicz E; Synthesis and biochemical properties of novel mRNA 5′ cap analogs resistant to enzymatic hydrolysis. Nucleosides Nucleotides & Nucleic Acids 24, 615-621

Jemielity J, Pietrowska-Borek M, Starzynska E, Kowalska J, Stolarski R, Guranowski A, Darzynkiewicz E; Synthesis and Enzymatic Characterization of Methylene Analogs of Adenosine 5′-Tetraphosphate (p4A). Nucleosides Nucleotides & Nucleic Acids 24, 589-593 (2005).

Stepinski J, Zuberek J, Jemielity J, Kalek M, Stolarski R, Darzynkiewicz E; Novel Dinucleoside 5′,5′-Triphosphate Cap Analogues. Synthesis and Affinity for Murine Translation Factor eIF4E. Nucleosides Nucleotides & Nucleic Acids 24, 629-633

Kalek M, Jemielity J, Stepinski J, Stolarski R, Darzynkiewicz E; A Direct Method for the Synthesis of Nucleoside 5′-Methylenebis(phosphonate)s from Nucleosides. Tetrahedron Letters 46, 2417-2421

 

2004

Lewdorowicz M, Yoffe Y, Zuberek J, Jemielity J, Stepinski J, Kierzek R, Stolarski R, Shapira M, Darzynkiewicz E; Chemical synthesis and binding activity of the trypanosomatid cap-4 structure. RNA 10, 1469-1478

Zuberek J, Jemielity J, Jablonowska A, Stepinski J, Dadlez M, Stolarski R, Darzynkiewicz E; Influence of electric charge variation at residues 209 and 159 on the interaction of eIF4E with the mRNA 5 ‘ terminus. Biochemistry 43, 5370-5379,

2003

Jemielity J, Fowler T, Zuberek J, Stepinski J, Lewdorowicz M, Niedzwiecka A, Stolarski R, Darzynkiewicz E, Rhoads RE. Novel “anti-reverse” cap analogs with superior translational properties. RNA 9, 1108-1122

Jemielity J, Stepinski J, Jaremko M, Haber D, Stolarski R, Rhoads RE, Darzynkiewicz E; Synthesis of novel mRNA 5 ‘ cap-analogues: Dinucleoside P-1, P-3-tri-, P-1, P-4-tetra-, and P-1, P-5-pentaphosphates. Nucleosides Nucleotides & Nucleic Acids 22, 691-694

Zuberek J, Jemielity J, Stepinski J, Lewdorowicz M, Niedzwiecka A, Haber D, Stolarski R, Rhoads RE, Darzynkiewicz E; Binding studies of eukaryotic initiation factor eIF4E with novel mRNA dinucleotide cap analogues. Nucleosides Nucleotides & Nucleic Acids 22, 1703-1706

Zuberek J, Jemielity J, Niedzwiecka A, Stepinski J, Wyslouch-Cieszynska A, Stolarski R, Darzynkiewicz E; Influence of the length of the phosphate chain in mRNA 5 ‘ cap analogues on their interaction with eukaryotic initiation factor 4E. Nucleosides Nucleotides & Nucleic Acids 22, 1707-1710 

Kiraga-Motoszko K, Stepinski J, Niedzwiecka A, Jemielity J, Wszelaka-Rylik M, Stolarski R, Zielenkiewicz W, Darzynkiewicz E; Interaction between yeast eukaryotic initiation factor eIF4E and mRNA 5 ‘ cap analogues differs from that for murine eIF4E. Nucleosides Nucleotides & Nucleic Acids 22, 1711-1714 

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